Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling

Huynh, Thu és Vörös, Mónika és Kedves, Orsolya és Turbat, Adiyadolgor és Sipos, György és Leitgeb, Balázs és Kredics, László és Vágvölgyi, Csaba és Szekeres, András (2022) Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling. MICROORGANISMS, 10 (2). pp. 1-13. ISSN 2076-2607

Szöveg microorganisms-10-00418.pdf Download (1MB)

Absztrakt (kivonat)

(1) Background: Bacillus velezensis and Bacillus amyloliquefaciens are closely related members of the “operational group B. amyloliquefaciens”, a taxonomical unit above species level within the ”Bacillus subtilis species complex”. They have similar morphological, physiological, biochemical, phenotypic, and phylogenetic characteristics. Thus, separating these two taxa from each another has proven to be difficult to implement and could not be pushed easily into the line of routine analyses. (2) Methods: The aim of this study was to determine whether whole FAME profiling could be used to distinguish between these two species, using both type strains and environmental isolates. Initially, the classification was determined by partial sequences of the gyrA and rpoB genes and the classified isolates and type strains were considered as samples to develop the identification method, based on FAME profiles. (3) Results: The dissimilarities in 16:0, 17:0 iso, and 17:0 FA components have drawn a distinction between the two species and minor differences in FA 14:0, 15:0 iso, and 16:0 iso were also visible. The statistical analysis of the FA profiles confirmed that the two taxa can be distinguished into two separate groups, where the isolates are identified without misreading. (4) Conclusions: Our study proposes that the developed easy and fast-automated identification tool based on cellular FA profiles can be routinely applied to distinguish B. velezensis and B. amyloliquefaciens.

Tudományterület / tudományág

műszaki tudományok > bio-, környezet- és vegyészmérnöki tudományok
természettudományok > biológiai tudományok

Kar

Nem releváns

Intézmény

Soproni Egyetem

Mű tipusa:	Cikk
SWORD Depositor:	Teszt Sword
Felhasználó:	Csaba Horváth
A mű MTMT azonosítója:	MTMT:32670309
Dátum:	14 Feb 2022 07:28
Utolsó módosítás:	14 Feb 2022 07:28
URI:	http://publicatio.uni-sopron.hu/id/eprint/2309

Actions (login required)

Tétel nézet